Isolation Frequencies of Cryphonectria parasitica (Murrill) and Other Fungi from Symptoms of Chestnut Blight in the Chestnut Forests of Artvin Province in Turkey

Yaşar Aksu¹ Berna Ç.Göktürk¹ Güven Aksu³ Himmet Tezcan²

           ¹Regional Directorate of Forestry-Artvin

           ² University of Uludag, Faculty of Agriculture, Department of Plant Protection-Bursa, Turkey

           ³ Science Institute of Artvın Coruh University

           Abstract

           A laboratory was established by Regional Directorate of Forestry of Artvin in 2014 to make biological control of Cryphonectria parasitica which damage chestnut forests in East Black Sea region. Isolates collected from wounded tissue with the symptoms of C. parasitica in areas where biological control protocols will be applied was brought to laboratory in 2014-2015. İsolates  were grown on PDA medium and isolates of C.parasitica with white colored (hypovirulent) and with orange colored(virulent) and other fungi were examined in terms of morphological characters. A total of 1794 parts with the symptoms of the disease in 329 petri plates The plant were planted. Among these isolated, 710 (%40.3) of them were developed as orange  colored C. parasitica, 459 (%26) of them developed as white colored C. parasitica, 297 (%16.8) of them developed as Penicillium spp, 29 (%1.6) of  them developed as Fusarium spp, 56 (%3.2 ) of them developed as Macrophomina spp. and 213  (%12.1)  of them developed as Trichoderma spp. According to Petri plates, 37 (%11.3) of 329 petri plates were orange colored C. parasitica, 29 (%8.8) petri plates were white colored C. parasitica, 7 (%2.1) petri plates were Trichoderma spp. and 257 (%78.1) petri plates were mixture of different fungi (orange colored C. parasitica, white colored C. parasitica, Trichoderma spp, Fusarium spp, Penicillium sp, Macrophomina spp, Chaetomium spp.). The most frequent isolates per petri plates with 40 petries (%12.2) was mixture of orange colored C. parasitica, white colored  C. parasitica and Penicillium spp.,  and followed by  mixture of orange colored C. parasitica and white colored C. parasitica with 37 petries (%11.3) and mixture of orange colored C. parasitica and Trichoderma spp. with 29 petri plates (%8.8). According to the field observation, although Trichoderma spp. were limited the proliferation of chestnut blight, it did not eradicates disease completely. However, isolates of white colored C. parasitica cured better the infections of orange colored C. parasitica.

           Keywords: Cryphonectria parasitica, Chestnut Blight, Artvin

            Yayın yeri: Aksu,Y; Ç.Göktürk,B,Ç; Aksu.G; Tezcan.H; Isolation Frequencies of Cryphonectria parasitica (Murrill) and Other Fungi from Symptoms of Chestnut Blight in the Chestnut Forests of Artvin Province in Turkey. 6 th International Chestnut Symposium October 2017 Ondokuz Mayıs University Samsun, Book of Abstracts Pages 80.