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Isolatıon, Characterızatıon and Pathogenıcıty of Fungı From Pristiphora abietina (Hymenoptera: Tenthredınıdae)
Yaşar AKSU tarafından
yazıldı
23 Nisan 2018 Pazartesi
Isolatıon, Characterızatıon and Pathogenıcıty of Fungı From
Pristiphora
abietina (Hymenoptera: Tenthredınıdae)
Nurcan Albayrak Iskender1* Serkan
Ortucu2 Yaşar Aksu3 Aysegul Saral4
1Department
of Nursing, Faculty of Health Sciences, Artvin Coruh University, Artvin, Turkey
2Department of Molecular Biology and Genetics,
Faculty of Science, Erzurum Technical University, Erzurum, Turkey
3Artvin Regional Forestry Management, Artvin, Turkey
4Department of
Nutrition and Dietetics, Faculty of Health Sciences, Artvin Coruh University,
Artvin, Turkey
Abstract
This study was
conducted to isolate and characterize pathogenic fungi for possible use in
biocontrol of P. abietina and to determine their pathogenicity. Eleven
fungal isolates were obtained from larvae. Based on traditional and molecular
methods, fungal isolates were identified as Beauveria bassiana, Penicillium
corylophilum, P. glabrum, P. polonicum, Aspergillus niger,
A. versicolor, Cladosporium sphaerospermum, Alternaria sp. and
Trichoderma asperellum. At first, all these fungal isolates were tested
against P. abietina larvae for isolate selection at 106 conidia/ml and
100% relative humidity (RH). Three fungal isolates ((PaF 004, PaF 009, PaF 076)
belonging to Beauveria genus were determined to be pathogen against P.
abietina. Then, mortality of these three isolates, on P. abietina larvae
were investigated at three conidial concentrations as 106, 107 and 108, and at
the degree of humidity as in the 65±5% and 100% RH, respectively. In 100% RH
and at 107–108 conidia/ml, three fungal isolates have shown a mortality rate of
100%. On the other hand, in 65±5% RH and at 107–108 conidia/ml, three fungal
isolates have shown a mortality rate between 49.1±4.52% - 91.1±7.2% and
63,3±4,5 - 95,3±2,7, respectively (p<0.05). The present results indicated
that PaF 009 has an excellent potential for biological control of P.abietina
larvae. This is also the first report on the larvacidal effect of B.
bassiana on P. abietina.
Keywords:
Pristiphora abietina, entomopathogenic fungi, Beauveria
bassiana, biological control Yayın yeri: Albayrak, İskender, N.; Örtücü ,S.; Aksu, Y.;
Saral,A.; Isolatıon, Characterızatıon
and Pathogenıcıty of Fungı From Pristiphora abietina (Hymenoptera:
Tenthredınıdae) Fresenius Environmental Bulletin , volume 26 – No.
1a/2017, pages 628-633
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