Olıgonychus ununguıs (Jacobı) (Acarı: Tetranychıdae)
Yaşar AKSU tarafından
yazıldı
23 Nisan 2018 Pazartesi
Isolatıon, Characterızatıon and Pathogenıcıty of
Bacterıa From Olıgonychus ununguıs (Jacobı) (Acarı: Tetranychıdae)
Nurcan Albayrak Iskender1*Yasar Aksu2
1 Faculty of
Health Sciences, Artvin Coruh University, 08000, Artvin, Turkey 2Artvin Regional
Forestry Management, 08000, Artvin, Turkey
Abstract
This study was
conducted to isolate and characterize pathogenic bacteria for possible use in
the biocontrol of Spruce Spider Mite Oligonychus ununguis and to
determine their pathogenicity. O. ununguis were collected from Savsat
Forests, in Artvin and transported to the laboratory to be used in the
experiments. According to the results of our study; eight bacteria species
belonging to seven genera of O. ununguis were isolated. Some morphological,
biochemical and other phenotypic properties of bacterial isolates were
determined by routine and conventional methods (API 20E, API 50 CH and API
Staph kits). In addition, 16S rRNA gene region also was sequenced. Based on
studies, bacterial isolates were identified as Methylobacterium sp. (Ou1),
Bacillus thuringiensis (Ou2), Staphylococcus chromogenes (Ou3), Pseudomonas
oleovorans (Ou4), Streptomyces sp. (Ou6), Paenibacillus apiarius (Ou7),
Mycobacterium sp. (Ou8) and Bacillus cereus (Ou9). All these
bacteria were tested against adults of O. ununguis within ten days. Bacillus
thuringiensis (Ou2) was found to be causing the highest pathogenicity
(40.7%).